Table 3 Primers used in PCRSeqTyping assay
From: Development of PCRSeqTyping—a novel molecular assay for typing of Streptococcus pneumoniae
Primers | Sequence (5′-3′) | Product size (in bp) | Serotype | ||
|---|---|---|---|---|---|
GROUP I | FP1 | CPS-1FP | GCAATGCCAGACAGTAACCTCTAT | 1061 | |
RP1 | CPS-2RP | CCTGCCTGCAAGTCTTGATT | |||
RP2 | 2538-RP | CTTTACCAACCTTTGTAATCCAT | 1109 | ||
GROUP II | |||||
HG1 | HG1-FP | FP1 | TGTCCAATGAAGAGCAAGACTTGAC | 1109 | 11A |
HG1-RP | RP1 | AAGTATATCCCTCCACAAACCCATC | 435 | 11D | |
316 | 18 F | ||||
HG2 | HG2-FP | FP1 | TGTCCAATGAAGAGCAAGACTTGAC | 1628 | 2 |
HG2-RP | RP5 | ATATCACTTTTTTACGGTAATGTCTA | 1820 | 41A | |
1185 | 7B | ||||
1820 | 40 | ||||
1819 | 9 V | ||||
1502 | 9A | ||||
HG3 | HG3-FP | FP1 | TGTCCAATGAAGAGCAAGACTTGAC | 1797 | 35 F |
HG3-RP | RP7 | CACCTTTATTTTCACTATCTGCATC | 1479 | 47 F | |
HG4 | HG4-FP | FP8 | ACTAGGAAGCTAGCCGTAGGTTGC | 366 | 22 F |
HG4-RP | RP8 | TCTCACCTTTAGTGCTTGAACCT | No Amplification | 22A | |
HG5 | HG5-FP | FP9 | CCATGGGATGCTTTCTGTGTGGA | 1061 | 10 F |
HG5-RP | RP9 | TATATCACTTTTTTACGGTAATGTCTA | 1004 | 10C | |
1416 | 11B | ||||
2958 | 11C | ||||
1395 | 13 | ||||
1395 | 20 | ||||
HG6 | HG6-FP | FP1 | TGTCCAATGAAGAGCAAGACTTGAC | 929 | 33 F |
HG6-RP | RP4 | AGCACCTAGCACCTGTTTAGAT | 929 | 33A | |
924 | 35A | ||||
927 | 35B | ||||
925 | 35C | ||||
HG7 | HG7-FP | FP3 | CAGAGTTCGTCTTACTTGGCAGCT | 737 | 34 |
HG7-RP | RP3 | GAATCTTGCAAGCTATTAATGATCG | 737 | 17A | |
HG8 | HG8-FP | FP6 | AGCAACTAGCCAAGTTAGCCAGAGT | 643 | 32 F |
HG8-RP | RP6 | ACTGTGCTTCCATCTGGGACATCATG | 648 | 32A | |
HG9 | HG9-FP | FP1 | TGTCCAATGAAGAGCAAGACTTGAC | 970 | 12 F |
HG9-RP | RP2 | CAGAAAAAGTAGCCTTATTTCTTAAGA | 996 | 44 | |
HG10 | HG10-FP | FP10 | ATGAAGCTATTCAAAGTTTGTTAGC | 656 | 25 F |
HG10-RP | RP10 | TGAATCCTCTAATCCTTGCATGA | 656 | 25A | |