Table 1 Primer and probe sequences for quantitative multiplex real-time PCR

ply-forward

5′-GCTTATGGGCGCCAAGTCTA-3′

78

NC_003028.3

ply-reverse

5′-CAAAGCTTCAAAAGCAGCCTCTA-3′

ply-probe

5′-Quasar 705-CTCAAGTTGGAAACCACGAGTAAGAGTGATGAA-3′-BHQ-2

lytA-forward

5′-ACGCAATCTAGCAGATGAAGCA-3′

75

NC_003028.3

lytA-reverse

5′-TCGTGCGTTTTAATTCCAGCT-3′

lytA-probe

5′-FAM-GCCGAAAACGCTTGATACAGGGAG-3′-BHQ-1

psoA-forward

5′-GCCCTAATAAATTGGAGGATCTAATGA-3′

114

U53509.1

psoA-reverse

5′-GACCAGAAGTTGTATCTTTTTTTCCG-3′

psoA-probe^a

5′-CAL Fluor Red 610-CTAGCACATGCTACAAGAATGATTGCAGAAAGAAA-3′-BHQ-2

Spn9802-forward

5′-AGTCGTTCCAAGGTAACAAGTCTAG-3′

157

AE005672.3

Spn9802-reverse

5′-ACCAACTCGACCACCTCTTTC-3′

Spn9802-probe^b

5′-CAL Fluor Orange 560-ATCAGATTGAAGCTGATAAAACGATAC-3′-BHQ-1 plus

GAPDH-forward

5′-GAAGGTGAAGGTCGGAGT-3′

226

BC083511.1

GAPDH-reverse

5′-GAAGATGGTGATGGGATTTC-3′

GAPDH-probe

5′-Quasar 670-CTCAAGTTGGAAACCACGAGTAAGAGTGATGAA-3′-BHQ-2