Standardisation and evaluation of a quantitative multiplex real-time PCR assay for the rapid identification of Streptococcus pneumoniae

Table 4 Sensitivity and specificity of quantitative multiplex real-time PCR (qmPCR)

Specimen	No. of samples	Culture positive for pneumococci (%)	Culture negative for pneumococci (%)	qmPCR positive^a (%)	qmPCR negative^b (%)
S. pneumoniae-culture positive isolates	43	43 (100)	0	43 (100)	0
Non-pneumococcal isolates	29	0	29 (100)	0	29 (100)
Spiked serum samples	30			30 (100)	0
Culture-positive serum samples	20	20 (100)	0	20 (100)	0
Culture-negative serum samples	26	0	26 (100)	0	26 (100)

^aSensitivity of qmPCR in pneumococcal culture-positive isolates and serum specimens was 100%
^bSpecificity of qmPCR in pneumococcal culture-negative isolates and serum specimens was 100%

ISSN: 2200-6133